KMID : 1134820120410081066
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Journal of the Korean Society of Food Science and Nutrition 2012 Volume.41 No. 8 p.1066 ~ p.1071
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Reduction of Allergic Potential of Meju by Three Step Fermentation
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Ryu Chung-Ho
Lee Jeong-Ok Son Dae-Yeul
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Abstract
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In this study we investigated the change in antigenicity and allergenicity of Meju, a traditional Korean soybean product, by fermentation via 3 different microorganisms. The steamed soybeans were fermented with Lactococcus lactis subsp. lactis and/or Aspergillus oryzae and/or Bacillus subtilis. Proteins in soybean were degraded after fermentation. Antigenicity or allergenicity were analysed by immunoblotting and ELISA using soybean protein-specific polyclonal antibodies or soybean allergic patient sera. The best degradation was achieved by three step fermentation using nisin-producing Lactococcus lactis subsp. lactis IFO12007, A. oryzae and B. subtilis. Allergenicity and antigenicity were also starkly reduced after three step fermentation. The three-step fermentation method developed in our lab suggests an excellent alternative to reduce the allergenicity of soybeans.
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KEYWORD
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allergic potential, fermentation, Meju, soybean
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